Berlin 2015 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 1: Imaging
BP 1.7: Vortrag
Montag, 16. März 2015, 11:30–11:45, H 1028
Helium Ion Microscopy of Biological Cells — •Natalie Frese1, André Beyer1, Matthias Schürmann2, Barbara Kaltschmidt2, Christian Kaltschmidt2, and Armin Gölzhäuser1 — 1Physics of Supramolecular Systems, University of Bielefeld, 33615 Bielefeld, Germany — 2Cell Biology, University of Bielefeld, 33615 Bielefeld, Germany
In this presentation HIM images of biological cells are presented. The presented study focuses on neuronal differentiated human inferior turbinate stem cells, mouse neurons and mouse fibroblasts. The cells were prepared by critical point drying and a flood gun was used to compensate charging, so no conductive coating was necessary.
Therewith, extremely small features at native cell surfaces were imaged with an estimated edge resolution of 1.5 nm. Due to the size of the structures and the preparation methods of the cells the observed features could be an indicator for lipid rafts. This hypothesis will be discussed.