Berlin 2015 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 13: Posters: Imaging and Superresolution Optical Microscopy
BP 13.7: Poster
Montag, 16. März 2015, 17:30–19:30, Poster A
In situ nanoscale imaging of chromosome oscillations in living yeast cells — •Natalja Strelnikova1, Vasily Zaburdaev2, Nora Sauter1, Manuel Guizar Sicairos3, Ana Diaz3, Petrina Delivani4, Mariola Chacon4, Iva Tolic-Norrelykke4,5, and Thomas Pfohl1 — 1Department of Chemistry, University of Basel, Switzerland — 2Max Planck Institute for the Physics of Complex Systems, Dresden, Germany — 3Paul Scherrer Institut, Villigen, Switzerland — 4Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany — 5Division of Molecular Biology, Institute Rudjer Boskovic, Zagreb, Croatia
Meiosis is a fundamental process in all eukaryotes leading to genetic diversity, but its details and the mechanism of homologous chromosome recombination is still poorly understood. Currently, there are no data on the change in total length and shape that chromatin undergoes during the oscillations before condensation into chromosomes. We employ a combined coherent scanning transmission X-ray microscopy (STXM) and ptychography setup, which allows us to investigate the dynamics of nuclear oscillations during meiosis of fission yeast Schizosaccharomyces pombe. We show that the combination of STXM and ptychography is an extraordinary tool to image living yeast cells during nuclear oscillation without additional labeling down to the submicron scale. Therefore we can analyze the chromatin compaction, mutual alignment of chromosomes and its impact on the recombination in eukaryotes on the relevant length scales of 30 to 100 nm.