Berlin 2015 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 16: Posters: Cell adhesion, mechanics and migration
BP 16.3: Poster
Montag, 16. März 2015, 17:30–19:30, Poster A
Growth-induced pressure of yeast populations — •Jörn Hartung1, Morgan Delarue2, and Oskar Hallatschek1,2 — 1MPI DS, Göttingen, Germany — 2UC Berkeley, CA, USA
Confined cells exert forces onto their surroundings during proliferation [1]. On the one hand these forces can redesign the population's microenvironment. In the case of microbes this can lead for instance to biofouling [2]. On the other hand these growth-induced forces imply a feedback onto the growing cells themselves, which can alter their morphology [3].
We designed a microfluidic device, which enables us to measure the growth-induced pressure confined S. cerevisiae populations exert onto a deformable PDMS membrane. Furthermore, we are able to control the chemical as well as the mechanical conditions the populations experience by employing nutrient channels and passive valves with different degrees of confinement, which we designed. The cells are subject to growth-induced steady state pressures ranging from 0.1 to 1.0 MPa.
[1] Markus Basan, Thomas Risler, Jean-François Joanny, Xavier Sastre-Garau and Jacques Prost, HFSP Journal (2009)
[2] T. Warsheid and J. Braams, Intl. Biodet. And Biodegrad. (2000)
[3] P. S. Stewart and C. R. Robertson, Applied microbiol. and biotech. (1989)