Berlin 2015 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 24: Posters: Membranes and vesicles

BP 24.5: Poster

Dienstag, 17. März 2015, 14:00–16:00, Poster A

UV induced Polymerization of Phospholipids at Langmuir Blodgett Through — •Roland Hillmann, Marius Dotter, Sören Grannemann, Lukas Galla, Andy Sischka, and Dario Anselmetti — Experimental Biophysics & Applied Nanoscience, Faculty of Physics, Bielefeld University, 33615 Bielefeld, Germany

We investigate the translocation of macromolecules like ssDNA through biological nanopores with Optical Tweezers force mechanics. Therefore Alpha-Hemolysin biopores are integrated into supported bilayer lipid membranes (BLM) or into polymerized lipid-bilayers. This will be accomplished either by the black lipid painting method, or by a liquid-solid Langmuir-Blodgett transfer with subsequent UV light polymerization of the phospholipids containing diacethylene. The high stability of the BLM allows an analyzation by AFM or HIM.