Berlin 2015 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Neurophysics II
BP 8.6: Vortrag
Montag, 16. März 2015, 16:00–16:15, H 1058
Electro-physiological characterization of the ultra-fast Channel-Rhodopsin Chronos — •Ulrich Fromme1, Andreas Neef2,3, and Christoph F. Schmidt1 — 1Faculty for Physics, George August University, Goettingen, Germany — 2Bernstein Center for Computational Neuroscience, Goettingen, Germany — 3MPI for Dynamics and Self-Organization, Goettingen, Germany
The light-gated ion channels Channel-Rhodopsins (CHRs) have become a major tool in experimental neuro science due to their low invasiveness and the possibility of genetically targeting specific cell types. To address specific issues, various ChRs with a wide array of different properties have been introduced. Here we characterize Chronos an especially light sensitive ChR with improved opening and closing speeds compared to traditional ChRs such as the widely used ChR2. This ChR shows great promise in various applications, such as the creation of neural implants, where good time resolution and low light intensities are favorable. We used patch-clamp recordings to characterize the kinetics of the ensemble of channels, which dictate the performance in applications. We also created Markov models representing the light cycle of Chronos, which enables the extraction of single-molecule properties from the electro-physiological measurements.