Heidelberg 2015 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

MO: Fachverband Molekülphysik

MO 4: Cold Molecules 1

MO 4.5: Vortrag

Montag, 23. März 2015, 15:30–15:45, PH/SR106

The Characterization of Complex Carbohydrates in Liquid Helium Nanodroplets using IR Spectroscopy — •Christiane Stachl¹, Johanna Hofmann¹, Heung Sik Hahm², Peter Seeberger², Kevin Pagel¹, and Gert von Helden¹ — ¹Fritz-Haber-Institut der MPG, Berlin, Germany — ²Max-Planck-Institut für Kolloid- und Grenzflächenforschung, Potsdam, Germany

Complex carbohydrates are typically characterized using mass spectrometry (MS)-based techniques. However, because the atomic composition of entire classes of monosaccharide building blocks is identical, it has proven difficult to differentiate the molecular structure of these biomolecules based on MS data alone. To overcome this fundamental problem, ion mobility-MS has been used to separate carbohydrates of identical mass but different structure according to their collision cross sections [1]. Conversely, it also is possible to use gas-phase infrared spectroscopy to gain additional information regarding the structure of complex carbohydrates. Ideal matrices in which to do this are liquid helium doplets, which are isothermal at 0.37 K, interact only weakly with an embedded molecule, and are transparent over a wide spectral range [2]. Here, we present the cold-ion IR spectroscopy of complex carbohydrates doped in liquid helium droplets using the Fritz-Haber-Institut free electron laser. We show that a nano-cryostat environment is an idyllic method to better resolve differences in the conformations of complex carbohydrates. [1] Pagel, K., Harvey, D. J., Anal. Chem., 2013, 85, 5138. [2] Toennies, J. P., Vilesov, A. F., Angew. Chem. Int. Ed., 2004, 43, 2622.