Hamburg 2016 – wissenschaftliches Programm
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ST: Fachverband Strahlen- und Medizinphysik
ST 2: Biomedical Imaging I
ST 2.8: Vortrag
Dienstag, 1. März 2016, 15:30–15:45, VMP6 HS C
Quantitative analysis of staining protocols for X-ray micro tomography — •Ronja Berg1, Madleen Busse1, Marian Willner1, Manuel Viermetz1, Melanie Kimm2, Alexander Fingerle2, Ernst J. Rummeny2, Franz Pfeiffer1, and Julia Herzen1 — 1Physics Department & Institute for Medical Engineering, Technical University of Munich, Munich, Germany — 2Radiology Department, Klinikum Rechts der Isar, Technical University of Munich, Munich, Germany
The limited soft-tissue contrast of the conventional micro computed tomography (microCT) can be overcome by staining protocols, which are increasing the atomic number and thus the contrast in the specimen. While various staining protocols exist, their exact influence on different types of tissue is still unclear. This fact makes it difficult to choose the best staining for different soft tissues, as the contrast gain can hardly be predicted. In this work we aim at studying two different staining protocols for soft tissue - one based on iodine and one on eosin. The stained soft-tissue was imaged using commercial microCT and phase-contrast tomography, which provides complementary and quantitative information. The imaging results were analyzed quantitatively in terms of Hounsfield Units in both modalities and compared to histopathology.