Hannover 2016 – wissenschaftliches Programm

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Q: Fachverband Quantenoptik und Photonik

Q 32: Biophotonics

Q 32.1: Vortrag

Mittwoch, 2. März 2016, 11:00–11:15, f342

Label-free imaging of single proteins and viruses ejected from a living cell — •Matthew McDonald, Katharina König, Marek Piliarik, Rui Qi Zhao, and Vahid Sandoghdar — Max Planck Institute for the Science of Light, Erlangen, Germany

A number of important physiological processes--such as cellular signaling and viral invasions--are marked by their secretomic behavior. Real-time, in-vivo sensing of these cellular secretion events commonly relies on labeled proteins and is thus inherently accompanied by the perturbation of the system. Here, we present a novel, label-free method that enables the detection of single protein secretions from living cells via an interferometric scattering technique (iSCAT). Secretomic events from an individual cell are imaged by way of iSCAT wherein single proteins and bioparticles from the kDa to MDa range are synchronously detected in real-time. The developed method has the potential to solve a wide range of problems in cellular physiology, such as intercellular signaling, immunology, and cancer malignancy.