Hannover 2016 – scientific programme
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Q: Fachverband Quantenoptik und Photonik
Q 39: Nano-Optics II
Q 39.2: Talk
Wednesday, March 2, 2016, 14:45–15:00, f342
Suppression of spontaneous Raman scattering for resolution improvement in label-free microscopy — •Steffen Rieger1, Klaus-Jochen Boller2, and Carsten Fallnich1,2 — 1Institute of Applied Physics, Westfälische Wilhelms-Universität Münster, Germany — 2MESA+ Institute for Nanotechnology, University of Twente, Enschede, The Netherlands
The electronic excitation of a complex molecule with UV light can lead to a significant change of its vibrational behavior and therefore to a heavily altered Raman spectrum. This effect can be used for the suppression of Raman scattering, which is a prerequisite for a STED-like resolution improvement in label-free microscopy [1].
We present first experimental results from our Resonance Raman Spectroscopy, which show already a suppression of specific Raman lines by up to 50 % relative to the background – an effect that is strong enough to provide a resolution improvement by more than a factor of two.
To achieve this, we currently use a spontaneous Raman scattering spectrometer with an excitation wavelength of 355 nm and pulse powers of up to 10 µJ to perform Resonance Raman Spectroscopy on the metal complex Tris(bipyridine)ruthenium(II) in Acetonitrile, which is known for its excited state Raman resonances.
[1] C. Cleff et al., Phys. Rev. A 86, 023825 (2012).