Hannover 2016 – wissenschaftliches Programm

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SYBO: Symposium Biomedical Optics

SYBO 2: Symposium Biomedical Optics 2

SYBO 2.1: Hauptvortrag

Donnerstag, 3. März 2016, 14:30–15:00, e415

Smart and multimodal light sheet microscopy — •Jan Huisken — Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Light sheet microscopy has become the tool of choice for in vivo fluorescence imaging of fragile biological specimens. Low phototoxicity and high speed acquisition have offered unique applications ranging from fast volumetric imaging to extended timelapse experiments spanning the entire embryogenesis of fish and fly. The enormous amounts of data from these instruments (TB/hour) now require a new approach to imaging: we cannot blindly image large samples with uniformly high spatial and temporal resolution.

We propose smart microscopy as a solution, in which illumination and detection are adaptively adjusted to obtain meaningful information at any time without any a priori knowledge about the sample. Only the regions in the sample that change over time or exhibit interesting new information will be illuminated and detected. Alternative modalities such as optical projection tomography offer additional information about the location and state of the sample that are used to optimize light sheet acquisitions. This presentation will give an overview of the latest developments in light sheet microscopy that lead towards a smart and gentle microscope.