Hannover 2016 – wissenschaftliches Programm

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SYBO: Symposium Biomedical Optics

SYBO 2: Symposium Biomedical Optics 2

SYBO 2.2: Hauptvortrag

Donnerstag, 3. März 2016, 15:00–15:30, e415

Laser Applications at the Cochlea: Imaging and Stimulation — •Alexander Heisterkamp^1,2, Nicole Kallweit^2,3, Peter Baumhoff⁴, Alexander Krueger^2,3, Nadine Tinne^2,3, Heiko Meyer^2,3, Andrej Kral^2,4, Hannes Maier^2,4, and Tammo Ripken^2,3 — ¹Institute of Quantum Optics, Leibniz University Hannover, Hannover, Germany — ²Cluster of Excellence Hearing for all, Hannover, Germany — ³Laser Zentrum Hannover e.V., Hannover, Germany — ⁴Institute of Audioneurotechnology, Hannover Medical School, Hannover, Germany

Using laser radiation, tissues and cells can be studied, characterized and manipulated with high resolution and low side effects. By optical tomography, single hair cells or their excitation can be visualized in model systems and even in vivo in an animal model. Using optoacoustics, sound generation within tissue can be induced. These processes can be employed for imaging or stimulation of the cochlea. In our investigations we studied the excitation of hair cells within the cochlea by laser irradiation and determined the underlying mechanisms. Further optical procedures allow the excitation of action potentials in neural cells as well and are currently studied with respect to applicability. For optical imaging, usually high resolution techniques such as confocal microscopy or histology are used to study the structure of the cochlea. However, optical penetration depth is limited by scattering within the osseous composition of the cochlea. Thus, we applied decalcification and optical clearing techniques to allow tomographic imaging of the cochlea ex vivo and generated 3D data of these structures.