Regensburg 2016 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 46: Posters - Biomaterials and Biopolymers

BP 46.1: Poster

Mittwoch, 9. März 2016, 17:00–19:00, Poster C

Neutron Reflectometry Yields Structural Insight into Protein Adsorption from Blood Serum onto Polymer Brushes — Ignacio Rodriguez Loureiro¹, •Victoria Latza¹, Avraham Halperin², Giovanna Fragneto³, and Emanuel Schneck¹ — ¹Max Planck Institute of Colloids and Interfaces, Potsdam, Germany — ²Université Joseph Fourier, Grenoble, France — ³Institut Laue-Langevin, Grenoble, France

The density profiles of proteins adsorbed from human blood serum onto poly(ethylene glycol) (PEG) brushes grafted to phospholipid surfaces are characterized by neutron reflectometry (NR). PEG brushes are commonly used to suppress undesired protein adsorption to biotechnological surfaces but brush failure is often reported. In contrast to conventional methods, NR with contrast variation allows directly distinguishing among primary protein adsorption at the grafting surface, secondary adsorption at the brush outer edge, and ternary adsorption to the polymer chains. We find significant primary protein adsorption into the lipid headgroup region. At the same time our results exclude pronounced ternary adsorption. Interestingly, the total amount of protein adsorbed to the brush-decorated surfaces is comparable to that adsorbed to the bare lipid surfaces.