Regensburg 2016 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 48: Posters - Bioimaging and Spectroscopy
BP 48.15: Poster
Mittwoch, 9. März 2016, 17:00–19:00, Poster C
Combined AFM/SPR ellipsometry study of protein adsorption at liquid/solid interface — •Peter Basa1, Peter Petrik2, and Nils Anspach1 — 1Semilab Semiconductor Physics Laboratory Co. Ltd., Budapest, Hungary — 2Institute for Technical Physics and Materials Science, Budapest, Hungary
The deeper understanding of interaction of biomaterials with solid surfaces is crucial in case of microfluidic devices applied to biosensorics. In this study, surfactant molecules mixed with protein species were adsorbed on gold nanofilm coated solid surfaces. Surface plasmon resonance (SPR) enhanced spectroscopic ellipsometry (SE) utilizing special adapted liquid cell, and atomic force microscopy (AFM) were used to study the adsorption dynamics, the resulting composition ratio of adsorbed species, and the biomolecule surface coverage depending on initial gold thin film surface structure/morphology.
In the poster, the performance of Semilab's combined SE and AFM measurement platform will be described using real experiment examples, with correlations pointed out in between different metrology results on the measured samples.