Regensburg 2016 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 48: Posters - Bioimaging and Spectroscopy
BP 48.2: Poster
Mittwoch, 9. März 2016, 17:00–19:00, Poster C
Aggregation of mono-stained proteins visualized ex vivo by two-dimensional polarization microscopy — •Daniela Täuber1, Rafael Camacho1, Christian Hansen2, Jia-Yi Li2, and Ivan Scheblykin1 — 1Chemical Physics, Lund University, Lund, Sweden — 2Biomedical Center, Lund University, Lund, Sweden
Neurodegenerative diseases are linked to aggregation of particular proteins. The investigation of pathologic pathways and the development of suitable medication demand for methods to visualize protein aggregation ex vivo. Sophisticated microscopy often requires two color labelling, while conventional fluorescence microscopy can reveal the expression of such proteins in brain tissue, but not their aggregation. Here we apply 2-dimensional polarization imaging [1] to visualize aggregation of human α-synuclein expressed in brain tissue from transgenic mice. We employ Förster Resonance Energy Transfer (FRET) between identical (single color) green fluorescent protein (GFP) tags linked to α-synuclein. We obtain information on aggregation, which cannot be seen from fluorescence intensity [3]. Our finding of α-synuclein aggregation in olfactory bulbs of old mice correlates with results from a behavioral study on that mice [2]. The aggregation pattern was not found in young mice.
D.T. acknowledges funding from the german science foundation DFG-TA 1049/1-1.
[1] Camacho, R. et al., Chem. Phys. 406, 30, 2012. [2] Hansen, C. et al., Neurobiol. Dis. 56, 145, 2013. [3] Camacho, R. et al., in preparation