Regensburg 2016 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 49: Posters - Cell Mechanics and Migration & Physics of Cancer

BP 49.12: Poster

Mittwoch, 9. März 2016, 17:00–19:00, Poster C

Mechanical properties of non-adhering cells — •Samaneh Rezvani¹, Tod M. Squires², and Christoph F. Schmidt¹ — ¹Drittes Physikalisches Institut, Georg-August-Universität, Göttingen, Germany — ²Department of Chemical Engineering, University of California, Santa Barbara, USA

Cells sense their micro-environment through biochemical and mechanical interactions. They can respond to stimuli by undergoing shape- and possibly volume changes. Key components in determining the mechanical response of a cell are the viscoelastic properties of the actomyosin cortex, effective surface tension, and the osmotic pressure. We use custom-designed microfluidic chambers with integrated hydrogel micro windows to be able to rapidly change solution conditions for cells without any hydrodynamic flow. We use biochemical inhibitors and different osmolytes and investigate the immediate response of individual cells. Using a dual optical trap makes it possible to probe suspended rounded-up cells by active and passive microrheology to quantify the response to the various stimuli.