Regensburg 2016 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 66: Biomaterials and Biopolymers III (Joint Session BP/CPP/MM)
BP 66.2: Vortrag
Donnerstag, 10. März 2016, 15:15–15:30, H45
Multiple bio-functionalization in 3D-scaffolds for cell manipulation realized by orthogonal (photo)chemistry — •Vincent Hahn1, Benjamin Richter2, Tanja Claus3,4, Guillaume Delaittre3,5, Christopher Barner-Kowollik3,4, Martin Wegener1,6, and Martin Bastmeyer2 — 1Institute of Applied Physics, Karlsruhe Institute of Technology (KIT) — 2Zoological Institute and Institute for Functional Interfaces, KIT — 3Institute for Chemical Technology and Polymer Chemistry, KIT — 4Institute for Biological Interfaces, KIT — 5Institute for Toxicology and Genetics, KIT — 6Institute of Nanotechnology, KIT
In recent years, we have applied Direct Laser Writing to fabricate 3D-microscaffolds for culturing cells in a well-defined environment and investigated cellular responses, e.g., contractility, adhesion and shape.
By sequential writing of different photoresists, patterned scaffolds are realized. They consist of protein-binding polymers next to regions containing light-activatable monomers in a non-protein binding background. Upon light-activation we were able to biotinylate specific regions in the passivating backbone. When incubated with a protein solution, proteins adsorb only onto protein-binding polymer areas. The biotin-linker is subsequently addressed by using avidin and any other biotinylated protein of choice. This technique has been successfully applied to fabricate scaffolds functionalized with two different adhesion proteins that selectively direct cell adhesion.
Such scaffolds might prove useful for applications in tissue engineering and stem cell differentiation.