Regensburg 2016 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 47: Poster: Interfaces and Thin Films
CPP 47.16: Poster
Mittwoch, 9. März 2016, 18:15–21:00, Poster B2
Human apolipoprotein A1 at water - solid and water - gas interfaces — •Susanne Dogan1, Michael Paulus1, Irena Kiesel2, and Paul Salmen1 — 1Fakultät Physik/DELTA, TU Dortmund, 44221 Dortmund, Germany — 2Institut Laue-Langevin, 71 avenue des Martyrs, 38000 Grenoble, France
The adsorption behaviour of human apolipoprotein A1 (Apo A1) at hydrophilic and hydrophobic interfaces was studied by means of X-ray reflectometry. Apo A1 is a transport protein and part of the high density protein (HDL) which is responsible for the transport of lipids, mainly cholesterol from the cell membranes to the liver. Due to its anisotropic surface, Apo A1 is able to interact with surfaces or interfaces via different interaction mechanisms including the hydrophobic and electrostatic interaction. pH dependent measurements at the solid - water interface between silicon dioxide and aqueous protein solution show that in a small pH range between pH 3 and pH 5 adsorption is increased due to electrostatic attraction. Here the native shape of the protein is altered, in order to maximize the contact with the interface. In contrast, the strong hydrophobic interaction between the protein and the liquid-gas interface yields the denaturation of the protein.