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UP: Fachverband Umweltphysik

UP 15: Methods - Measurement Techniques

UP 15.2: Talk

Thursday, March 10, 2016, 15:30–15:45, H41

Correlative Microscopy at ProVIS - Centre for Chemical Microscopy — •Matthias Schmidt, Hryhoriy Stryhanyuk, Hans Richnow, and Niculina Musat — Helmhotlz-Centre for Environmental Research (UFZ) Leipzig, Germany

The UFZ has established the ProVIS - Centre for Chemical Microscopy, recently. ProVIS aims to visualise biochemical processes on cellular level. It comprises of equipment for sample preparation, a number of different microscopes - fluorescence, electron (SEM)- and helium ion microscopes (HIM) and imaging mass-spectrometers. We will present a correlative microscopy study on an environmental sample of microbes and sediments taken from the Davidschacht mine-tailings. The sample was filtrated on a Pd/Au sputter-coated filter, chemically fixed, fluorescence in-situ hybridised (FISH) and critical-point-dried. In order to detect the positions of bacteria on the filter fluorescense microscopy was employed and the regions of interest (ROIs) were laser-marked. In the SEM and HIM providing a lateral as well as depth resolution of significantly better than 4nm the morphology of the bacteria detected by FISH was investigated. The chemical composition of the sediments in the sample was investigated by energy dispersive X-ray spectroscopy. Further chemical information was gained from nanoSIMS experiments. The results obtained by the different methods were correlated and overlayed using the ImageJ-plugin CORRELIA which we have developed at ProVIS.