Dresden 2017 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Posters - Single Molecule Biophysics

BP 10.2: Poster

Montag, 20. März 2017, 17:30–19:30, P3

Plasmonics and Nanofluidics for DNA-Single Molecule Detection — •Parisa Bayat, Irene Fernandez-Cuesta, Franziska Esmek, Thomas Kling, and Robert H. Blick — Center for Hybrid Nanostructures & Institute of Nanostructure- and Solid State Physics, University of Hamburg, Germany

Plasmonic antenna nano-focus the light beyond diffraction. These hot spots are ultra-sensitive, what can be exploited for single molecule (bio) sensing. But there is a major challenge: placing the target element at the sensitive area. Here, we have integrated a sub-100nm nanochannel crossing the antenna gap, what allows the in-line detection of single molecules of DNA in real-time as they pass through the light "hot-spot". In this configuration, the molecules are detected as peaks in the fluorescent signal in time scans. This allows real time read-out of the molecules with no limitation in the length and without an expensive camera. For total liquid control, the nanochannel is connected to a complete fluidic system. This represents a new type of super-sensitive (bio) sensor, with single- molecule real time detection capabilities. We have developed a wafer-scale fabrication process, based on nanoimprint lithography [1], to make the complete fluidic devices in one single step, only 120 seconds. Discrete DNA molecules have been detected and counted by in-line detection in real time. Different types of viral DNA molecules (λ-Bacteriophage and Kaposi's sarcoma herpesvirus) were stained with intercalating dyes and stretched in the nanochannels.

[1] I. Fernandez-Cuesta et al., J. Vac. Sci. Technol. B29, 06F801 (2011)