Dresden 2017 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 2: Bioimaging and Spectroscopy I
BP 2.2: Vortrag
Montag, 20. März 2017, 10:00–10:15, HÜL 386
(3+1)D SIM + quantitative analysis for ophthalmologic research — •Florian Schock1,2,3, Gerrit Best1,3, Nil Celik4, Yangyi Wang2,3, Alena Bakulina5, Saadettin Sel4, Udo Birk1,3, Rainer Heintzmann6,7, Jürgen Hesser5, Stefan Dithmar4,8, and Christoph Cremer1,2,3 — 1Institute of Molecular Biology, University of Mainz — 2Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg — 3Kirchhoff Institute for Physics, University of Heidelberg — 4Department of Ophthalmology, University-Hospital Heidelberg — 5Experimenatal Radiation Oncology, University Medical Center Mannheim, University of Heidelberg — 6Institute of Physical Chemistry and Abbe Center of Photonics, University of Jena — 7Leibniz Institute of Photonic Technology — 8Department of Ophthalmology, HELIOS HSK, Wiesbaden, Germany
While super-resolution-microscopy has become widely available, its application in clinical context is still mostly restricted to cultivated cells. We present the application of (3+1)D(3 excitation/emission spectra) Structured Illumination Microscopy to clinical research on extracted tissues as well as quantitative analysis of over 300 human RPE cells and their granules (intracellular particles) regarding connections to age related macular degeneration. Autofluorescencence behaviour is a characteristic hallmark for several chorioretinal conditions, so SIM offers potential for further clinical imaging at illumination powers that allow application to living patients (ongoing clinical study).