Dresden 2017 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 2: Bioimaging and Spectroscopy I
BP 2.8: Vortrag
Montag, 20. März 2017, 12:00–12:15, HÜL 386
A scanning ion conductance microscope (SICM) for large-range imaging — •Nicolas Schierbaum, Martin Hack, and Tilman E. Schäffer — Institute of Applied Physics, University of Tübingen, Auf der Morgenstelle 10, 72076 Tübingen, Germany
The scanning ion conductance microscope (SICM) is a high-resolution imaging technique using an electrolyte-filled nanopipette as probe, allowing non-invasive, contact-free topography imaging of soft and fragile biological samples in physiological conditions. The scan range of previously described SICM setups is limited to 200 µm in lateral and 25 µm in vertical direction. We present a SICM setup with a maximum scan range of 25 mm × 25 mm in lateral and 0.25 mm in vertical direction. The SICM is combined with an optical phase contrast microscope and is equipped with a heated sample stage for live cell imaging. We demonstrate the high versatility of the setup by imaging samples at different length scales: from macroscopic structures such as fingerprints or cell layers to microscopic structures such as small cell protrusions. The large scan range facilitates selecting a region of interest with subsequent high-resolution imaging. We applied the setup to the study of wound healing by time-lapse imaging a live epithelial cell monolayer over 20 hours, demonstrating the long-term imaging stability of the setup.