Dresden 2017 – wissenschaftliches Programm
BP 21.13: Poster
Dienstag, 21. März 2017, 14:00–16:00, P1A
Cancer specific plasma membrane association of Hsp70-1A - AFM and fluorescence imaging of model membranes — •Constanze Lamprecht1, Josef Madl2, 3, Winfried Römer2, 3, Mathias Gehrmann4, and Andreas Ebner1 — 1Institute of Biophysics, Johannes Kepler University Linz, Austria — 2Centre for Biological Signalling Studies (BIOSS) , University Freiburg, Germany — 3Faculty of Biology, University Freiburg, Germany — 4Klinikum rechts der Isar, TU Munich, Germany
Hsp70A1A is the major stress-inducible member of the HSP70 chaperone family and has been implicated in cancer diseases with the development of tumor resistances to standard therapies, increased invasiveness and poor prognosis. In normal cells Hsp70A1A is expressed in response to external stimuli such as physical exertion and heat to deal with denatured proteins and prevent toxic aggregations. In a majority of human tumors the protein is produced permanently in high amounts and a significant fraction of the cytosolic protein is found associated with cellular membranes. In this work we study the cancer specific plasma membrane localization of Hsp70A1A. As the protein lacks a consensus sequence for translocation to the cell membrane as well as known membrane binding domains, its anchorage may follow a new paradigm of protein-lipid interactions that may hold the key for deciphering membrane associated functions of Hsp70A1A in cancer evolution. We conduct AFM investigations in combination with fluorescence microscopy on model membranes to determine the anchoring mechanism and function on the cell surface.