Dresden 2017 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 29: Posters - Multi-Cellular Systems
BP 29.12: Poster
Dienstag, 21. März 2017, 14:00–16:00, P2-EG
Protein-protein interactions in developmental cell-cell fusion quantified by fluorescence fluctuation spectroscopy — •Valentin Dunsing1, Benjamin Podbilewicz2, and Salvatore Chiantia1 — 1Cell Membrane Biophysics, Institut für Biochemie und Biologie, Universität Potsdam — 2Department of Biology, Technion - Israel Institute of Technology, Haifa
Cell-cell fusion is a universal process in development which is involved in the formation of various organs and tissues. Failure of fusion leads to severe morphogenic disorders. The epithelial fusion failure protein EFF-1 was shown to be necessary and sufficient for fusion in the small nematode C.elegans and cell culture systems. However, its mechanism of action remains poorly understood.
Using fluorescence fluctuation microscopy approaches (Number&Brightness, Scanning FCS) we study the cis- and trans- interactions of EFF-1 and its dynamics in the plasma membrane. We observe a concentration dependent cis-trimerization of the protein in living cells and monitor cell-cell (i.e. protein-protein) trans-interactions by calculating cross-correlation of spectrally separated fluctuations.
Finally, we present an experimental setup to investigate EFF-1 interactions in vivo, i.e. epithelial cell-cell fusions in developing C.elegans embryos.