Dresden 2017 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 55: Cell Migration and Contraction

BP 55.1: Hauptvortrag

Donnerstag, 23. März 2017, 15:00–15:30, HÜL 386

Network heterogeneity regulates steering in actin-based motility — •Laurent Blanchoin — CytoMorphoLab, Biosciences & Biotechnology Institute GRENOBLE, FRANCE

The growth of branched actin networks powers cell-edge protrusions and motility. These dynamic structures are characterized by a heterogeneous actin density, which yields to a tunable cellular response. We studied how actin organization controls both the rate and the steering during lamellipodium growth. We used a high-resolution surface structuration assay combined with modeling approach to describe the growth of a reconstituted lamellipodium. We demonstrated that local monomer depletion at the site of active assembly negatively impacts the network growth rate. At the same time, network architecture tunes the protrusion efficiency, and regulates the rate of growth. One consequence of this interdependence between monomer depletion and network architecture effects is the ability of heterogeneous network to impose steering during motility. We established therefore the general principles on how the cell can modulate the rate and the direction of protrusion locally by varying both density and architecture of its actin network.