Dresden 2017 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 55: Cell Migration and Contraction

BP 55.4: Vortrag

Donnerstag, 23. März 2017, 16:00–16:15, HÜL 386

Combined MEA- and SICM-based measurements of the cardiomyocyte contractile behavior — •Stefan Simeonov and Tilman E. Schäffer — Institute of Applied Physics, University of Tübingen, Germany

We present a novel experimental setup that combines microelectrode array (MEA) based electrical recording with scanning ion conductance microscopy (SICM). MEAs are used for non-invasive, in-vitro measurements of extracellular electrophysiological signals generated by live cells. SICM is a non-invasive scanning probe technique for imaging sample topography with sub-micrometer spatial resolution. We used this combined setup to investigate beating cardiac cells (HL-1 cell line), which exhibit both electrophysiological activity and morphological dynamics. We mapped the height of a beating cell as a function of time with sub-cellular spatial and millisecond temporal resolution. Synchronizing the SICM data with the simultaneously recorded extracellular field potential from the MEA allowed us to reconstruct the time-resolved 3D topography during the contraction and relaxation cycle of the cell. Furthermore, we investigated the effect of blebbistatin, a myosin II motor protein inhibitor, on the contractile behavior of beating cells. The contraction amplitude decreases over time until the cells stop contracting entirely despite the ongoing generation of unaltered extracellular field potential.