Dresden 2017 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 7: Posters - Mechanics and Dynamics of 3D Tissues (Focus Session)

BP 7.7: Poster

Montag, 20. März 2017, 17:30–19:30, P3

Organs-on-a-chip: Microphysiological platforms as in vitro models of cardiac and adipose tissue — •Oliver Schneider, Julia Rogal, Christopher Probst, and Peter Loskill — Department of Cell and Tissue Engineering, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart, Germany

Drug discovery and development to date relies on animal models, which are useful, but fail to resemble human physiology. The discovery of human induced pluripotent stem (iPS) cells has led to the emergence of drug screening using human disease-specific organ-models. One promising approach are microfluidic devices, simulating 3D tissue structure and function. Using microfabrication techniques we have developed two microphysiological platforms (MPSs) incorporating in vitro models of human cardiac and adipose tissue. Both MPSs consist of three functional components: a tissue culture chamber mimicking organ-specific geometrical in vivo properties; 'vasculature-like' media channels enabling a precise delivery of compounds (nutrients, drugs); *endothelial-like* barriers protecting the tissues from shear forces while allowing diffusive transport. We developed and deployed a novel biocompatible membrane as barrier, matching the desired organ-specific properties by varying its porosity. Both organ-chips manage to create physiological micro-tissues that are viable and functional for multiple weeks. The developed chips are the first systems that combine human genetic background, physiologically relevant tissue structure and 'vasculature-like' perfusion. Both MPSs are extremely versatile and can be applied for drug toxicity screening and mechanistic research on tissue dynamics.