Dresden 2017 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 8: Posters - Bioimaging and Spectroscopy
BP 8.14: Poster
Montag, 20. März 2017, 17:30–19:30, P3
PDMS micro-moulds as test samples for Scanning Ion Conductance Microscopy — Annelie Marx1, •Regina Lange1, Henrike Rebl2, Barbara Nebe2, Ingo Barke1, and Sylvia Speller1 — 1University of Rostock, Institute of Physics, 18059 Rostock — 2University Medical Center Rostock, Dept. of Cell Biology, 18057 Rostock
Scanning Ion Conductance Microscopy (SICM) is a less known scanning probe method that uses a nanopipette with an opening diameter below 100 nm as probe. The topography of a soft non-conducting material placed in a (conducting) liquid is measured on the nanoscale, avoiding direct forces between the sample and the probe. Hence, SICM represents a good choice for in vitro high resolution imaging of living cells in physiologic medium. Therefore, among others, the influence of the substrate structure and of potential, or of light on the cell behaviour can be studied in real time.
For testing the SICM method and for making available prospective substrates for cell adhesion experiments we prepare PDMS (polydimethylsiloxane) micro-moulds of various samples. For instance moulds of dry etched glass structures exhibiting equidistant grids and pillars of different aspect ratios with vertical side walls are produced. To compare the replica with their original both were studied by AFM. A decent reproduction quality was obtained with 2 micrometer deep grooves and unity aspect ratio.