Dresden 2017 – wissenschaftliches Programm
CPP 14.4: Poster
Montag, 20. März 2017, 18:30–21:00, P1C
AFM study of evaporated phospholipidic bilayer on thin film chitosan in liquid environment — Rodrigo Catalan1,3, Maria Jose Retamal1,2,3, Diego Diaz1,3, Marcelo Cisternas1,3, Nicolas Moraga1,3, Tomas P. Corrales4, Marco Soto-Arriaza2, Patrick Huber5, and •Ulrich G. Volkmann1,3 — 1Instituto de Fisica, P. Univ. Catolica de Chile (UC), Santiago, Chile — 2Facultad de Quimica, P. Univ. Catolica de Chile (UC), Santiago, Chile — 3CIEN-UC, P. Univ. Catolica de Chile (UC), Santiago, Chile — 4Facultad de Fisica, UTFSM, Valparaiso, Chile — 5Institute of Materials Physics and Technology, TUHH, Hamburg, Germany
Self-assembly of artificial biological membranes on solid substrates has gained importance due to the potential applications in the field of BioNanotechnology. Particularly important are phospholipidic membranes, e.g. DPPC, which interact with proteins that regulate the flow of ions across the membrane. In a previous work we have shown that DPPC forms a bilayer on silicon, which can serve for biosensoric applications. We have extended this concept to other phospholipids to explore their interactions with proteins. We present a thermal and morphological study of three phospholipidic thin-films: DODAB, DSPC and DMPC. These films are fabricated by Physical Vapour Deposition (PVD) on silicon and analyzed with Atomic Force Microscopy (AFM) in liquid at various temperatures. We show that these phospholipids do not decompose during PVD. Acknowledgements: MJR: Fondecyt postdoc 3160803. UGV: Fondecyt 1141105 y CONICYT-PIA ACT 1409. TPC: Fondecyt Iniciación 11160664, MC: CONICYT scolarship.