Dresden 2017 – wissenschaftliches Programm
CPP 18.10: Poster
Montag, 20. März 2017, 18:30–21:00, P1C
Phase behavior of dense lysozyme and nanoparticle solutions — •Julian Schulze1, Nico König1, Karin Julius1, Steffen Bieder1, Michael Paulus1, Julia Nase1, Metin Tolan1, and Roland Winter2 — 1Fakultät Physik/Delta, TU Dortmund, 44221 Dortmund, Germany — 2Fakultät für Chemie und Chemische Biologie, TU Dortmund, 44221 Dortmund, Germany
In previous studies, small-angle X-ray scattering was used to study the intermolecular interaction potential of lysozyme solutions under the influence of varying environmental conditions such as protein concentration, c, temperature, T, and pressure, p. The attractive part of the interaction potential exhibits a non-monotonous p-dependence with a minimum at ~ 2 kbar at selected temperatures. Adding NaCl leads to more prominent attractive short range interactions, especially at high c and low T, and the homogeneous protein solution becomes turbid due to the formation of a metastable liquid-liquid phase separated (LLPS) state, where lysozyme forms small droplets of high concentration within a more dilute liquid phase. Due to depletion attraction, Ludox silica nanoparticles show a liquid-liquid phase separation as well when polymers are added. Here, we discuss differences in the mechanism and phase behavior of these dense lysozyme and nanoparticle solutions.