Dresden 2017 – wissenschaftliches Programm
CPP 18.4: Poster
Montag, 20. März 2017, 18:30–21:00, P1C
Influence of added crosslinker on the kinetics of LLPS studied by USAXS — •Richard Schäufele1, Stefano Da Vela1, Johannes Möller2, Fajun Zhang1, and Frank Schreiber1 — 1Institut für Angewandte Physik, Universität Tübingen, 72076 Tübingen — 2ESRF, Grenoble, France
In the presence of trivalent cations, e.g. Y3+, BSA solutions feature a liquid-liquid phase separation (LLPS) with a lower critical solution temperature.
During LLPS the dense phase of the system can be brought to a dynamically arrested state before reaching the binodal for temperatures below denaturation.
By adding a bifunctional crosslinker, Glutaraldehyde, protein monomers form clusters based on inherently multiple reaction sites.
Crosslinker concentration directly impacts size and size distribution of protein clusters, altering the binodal as well as the glass line.
In order to understand the influence of oligomerization on the kinetics of LLPS and the arrested state, dense phases with different crosslinker concentrations are studied.
The experiments focus on the kinetics during phase separation for different temperature quenches. By
using time-resolved ultra small angle X-ray scattering (USAXS) complemented with DLS, the evolution of the characteristic length can be followed through the different stages of the phase separation.
Surprisingly, an increasing crosslinker concentration does not display a monotonic trend considering the characteristic length. The latter as well as the behaviour of intensity and arrested state will be discussed with respect to the temporal evolution.
Stefano Da Vela, et al., Soft Matter, 2016, 12, 9334-9341