Dresden 2017 – wissenschaftliches Programm
CPP 2.3: Vortrag
Montag, 20. März 2017, 10:30–10:45, SCH A251
In vivo quantification of spatially-varying mechanical properties in developing tissues — •Friedhelm Serwane1,2, Alessandro Mongera1, Payam Rowghanian1, David Kealhofer1, Adam Lucio1, Zachary Hockenbery1, and Otger Campas1 — 1University of California, Santa Barbara, USA — 2Max Planck Institute for Medical Research, Heidelberg, Germany
We present a technique that allows quantitative spatiotemporal measurements of mechanical properties in vivo using biocompatible ferrofluid droplets as local mechanical actuators .
The mechanical properties of the cellular microenvironment and their spatiotemporal variations play a central role in controlling cell behavior, including cell differentiation. However, no direct in vivo and in situ measurement of mechanical properties within developing 3D tissues has been performed yet.
Using our technique we show that vertebrate body elongation entails spatially varying tissue mechanics along the anteroposterior axis. Specifically, we find that the zebrafish tailbud is viscoelastic: elastic below a few seconds and fluid after just one minute. Furthermore, it displays decreasing stiffness and increasing fluidity towards its posterior elongating region.
This method opens the door to study mechanobiology in vivo, both in embryogenesis and in disease processes, including cancer.
F. Serwane, A. Mongera, P. Rowghanian, D. Kealhofer, A. Lucio, Z. Hockenbery, O. Camp‘as. Nature Methods, in press (2016)