Dresden 2017 – wissenschaftliches Programm
CPP 26.5: Vortrag
Dienstag, 21. März 2017, 15:00–15:15, ZEU 260
Stained microparticles for lifetime encoding — •Daniel Kage1, Linn Fischer1, Katrin Hoffmann1, Marc Wittkamp2, Jens Ameskamp2, Wolfgang Göhde2, Thomas Thiele3, Uwe Schedler3, and Ute Resch-Genger1 — 1Bundesanstalt für Materialforschung und -prüfung (BAM), Richard-Willstätter-Str. 11, 12489 Berlin — 2Quantum Analysis GmbH, Mendelstr. 17, 48149 Münster — 3PolyAn GmbH, Rudolf-Baschant-Str. 2, 13086 Berlin
Multiparametric analyses involving optical techniques like flow cytometry are at the core of studying complex systems in biological research and diagnostic applications . However, for fluorescence-based techniques, the number of reporters distinguishable in spectral multiplexing is limited by spectral overlap and requires a multitude of excitation light sources and detection systems . Intensity encoding often used for bead assays suffers from problems regarding dye concentration control and excitation light intensity fluctuations . An alternative is luminescence lifetime encoding , particularly to minimize instrument costs. Here, we report on dye-stained polymer microparticles for lifetime encoding in flow cytometry with different organic dyes. This ranges from studies of the impact of parameters like dye loading concentration and particle diameter on fluorescence decay behavior to the demonstration of lifetime code reading and simultaneous ligand fluorescence signal detection with single-wavelength excitation in a flow.
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