Dresden 2017 – wissenschaftliches Programm
CPP 4.2: Vortrag
Montag, 20. März 2017, 10:45–11:00, ZEU 114
Laser scanning microscopy as method to investigate on surface-liquid-interaction — •Frank Schellenberger, Alexander Saal, Martin Treß, Periklis Papadopoulos, Tadashi Kayija, Doris Vollmer, and Hans-Jürgen Butt — Max Planck Institute for Polymer Research, Mainz, Germany
Laser scanning confocal microscopy (LSCM) is widely used in biological and medical research for the three-dimensional imaging of cells and subcellular structures. However, the capabilities of LSCM have been hardly exploited in imaging wetting and interfacial phenomena. Wetting plays an important role in a wide variety of technological, biological, and environmental processes. Currently, a lot of research is devoted to controlling wetting via superhydrophilic or superhydrophobic surfaces. Understanding the interplay between microscopic structure and macroscopic wetting properties is necessary for the optimization of these surfaces. Inverted LSCM are ideally suited to analyze is wetting of structured surfaces because it allows to observe the important solid-liquid interface at high resolution.
LSCM can operate in two modes; fluorescence, which requires labelling of the structures to be imaged with fluorescent dyes, and reflection mode, where the interfaces can be observed directly. The general operating principles of LSCM and an overview with examples of the application will be presented.