Dresden 2017 – wissenschaftliches Programm
CPP 4.5: Vortrag
Montag, 20. März 2017, 11:45–12:00, ZEU 114
Vesicles-on-a-chip : A universal microfluidic platform for the assembly of liposomes and polymersomes — •Julien Petit1, Ingmar Polenz1, Laura Thomi2, Frederik Wurm2, Jean-Christophe Baret3, Katharina Landfester2, Stephan Herminghaus1, and Oliver Bäumchen1 — 1Max Planck Institute for Dynamics and Self-Organization (MPI-DS), 37077 Göttingen, Germany — 2Max Planck Institute for Polymer Research (MPI-P), 55128 Mainz, Germany — 3Centre de Recherche Paul Pascal (CRPP), CNRS, Université de Bordeaux, 33600 Pessac, France
One key challenge nowadays for a "bottom-up" approach in synthetic biology relies on the fabrication of compartments such as vesicles that can be viewed as model membranes. Nevertheless, the development of reliable methods for the high-throughput production of vesicles in an easy and well-controlled manner is still in progress. In this context, we propose a versatile method for producing monodisperse liposomes as well as polymersomes on the exact same PDMS-based microfluidic platform from double-emulsions [J. Petit et al., EPJE 39: 59 (2016)]. The size of the vesicles obtained with this technique can be varied over at least one order of magnitude and they are stable for more than 3 months under ambient conditions. Furthermore, we demonstrate the versatility of this microfluidic platform by producing polymersomes composed of functionalized block-copolymers. We characterize the successful functionalization by fluorescent labeling and measure the specific adhesion of polymersomes on dedicated surfaces using a micropipette force sensor technique.