Dresden 2017 – wissenschaftliches Programm
CPP 52.10: Poster
Mittwoch, 22. März 2017, 18:30–21:00, P3
Smart Binary Polymer Brush Surfaces For Controlled Biocatalysis — •Alice Rosenthal1,2, Sebastian Rauch1, Klaus-Jochen Eichhorn1, Manfred Stamm1, and Petra Uhlmann1,3 — 1Leibniz-Institut für Polymerforschung Dresden e.V., PF 120411, 01005 Dresden — 2Technische Universität Dresden, 01062 Dresden — 3Department of Chemistry, Hamilton Hall, University of Nebraska-Lincoln, 639 North 12th Street, Lincoln, Nebraska 68588, United States
Polymer brushes represent smart surfaces due to their ability to respond to changes in the environmental conditions (e. g. temperature) by showing a reversible swelling-deswelling behavior. Moreover, polymers with functional groups offer a way to immobilize enzymes in an active manner. By combining polymers with opposite properties, switching between different states depending on the external conditions becomes feasible. We fabricated binary polymer brushes consisting of the Poly(N-isopropylacrylamide) (PNIPAAm) and Poly(ethylene glycol) (PEG). In contrast to PEG, PNIPAAm exhibits a lower critical solution temperature (LCST) close to the physiological range (31 °C). In-situ spectroscopic ellipsometry was used to investigate the temperature-responsive swelling behavior of the binary brushes in comparison to the respective homo-brushes. Horseradish Peroxidase conjugated Streptavidin (SA-HRP) was immobilized through accessible alkyne groups at the outer extremity of the PNIPAAm chains. For the enzyme modified binary PNIPAAm/PEG brushes, the enzymatic activity can be switched between an active state at 20°C and a less active state at 40°C in aqueous medium.