Dresden 2017 – wissenschaftliches Programm
CPP 52.3: Poster
Mittwoch, 22. März 2017, 18:30–21:00, P3
Polymer microgels in cell-free biotechnology — •Max Männel, Nicolas Hauck, Thomas Heida, Ivan Raguzin, Anika Kaufmann, Andreas Fery, and Julian Thiele — Leibniz-Institut für Polymerforschung Dresden e. V., Leibniz Research Cluster, Germany
The formation of microemulsions in microfluidic flow cells, termed droplet microfluidics, has been established as highly modular method for the fabrication of polymer microgels. The microfluidic flow cells are commonly fabricated by a combination of photo- and soft lithography using poly(dimethylsiloxane) (PDMS). A promising alternative which is less expensive and time-consuming is additive manufacturing, also referred to as 3D printing, which allows the fabrication of flow cells in an affordable, high-resolution and fast single-step process. Our investigated polymer microgels are formed by crosslinking thiol-functionalized hyaluronic acid (HASH) and poly(ethylene glycol) diacrylate (PEGDA) via mild thiol-Michael addition. These microgels have various applications in cell-free biotechnology since they provide a controllable reaction environment. One topic of intense research in our group is the immobilization of enzymes for the production of novel, non-natural pharmaceuticals, e. g. polyketides. Another relevant process in biotechnology is the cell-free protein synthesis which can be realized by attaching DNA to the same polymer microgels. Performing these reactions in microfluidic bioreactors with various reaction chambers and a continuous flow allow the production of seldom products which can not be fabricated by conventional processes.