Dresden 2017 – wissenschaftliches Programm
DY 53.10: Poster
Donnerstag, 23. März 2017, 17:00–19:30, P1A
Highly Efficient Multivalent 2D Nanosystems for Inhibition of Orthopoxvirus Particles — Benjamin Ziem1, Hendrik Thien2,3, Katharina Achazi1, Constanze Yue3, Daniel Stern3, Kim Silberreis3, •Mohammad Fardin Gholami4, Fabian Beckert5, Dominic Gröger1, Rolf Mülhaupt5, Jürgen P. Rabe4, Andreas Nitsche3, and Rainer Haag1 — 1Institute of Chemistry and Biochemistry, Freie Universität Berlin — 2Institute of Virology, University of Leipzig — 3Robert Koch Institute, Center for Biological Threats and Special Pathogens, Berlin — 4Department of Physics & IRIS Adlershof, Humboldt-Universität zu Berlin — 5Institute for Macromolecular Chemistry, University of Freiburg
Efficient inhibition of cell-pathogen interaction to prevent subsequent infection is an urgent but yet unsolved problem. In this study, we developed a new 2D multivalent surface, functionalized with sulfated dendritic polyglycerol (dPG), to enable virus interaction. A simple "graft from" approach enhances the solubility of thermally reduced graphene oxide (TRGO) and provides a suitable 2D surface for multivalent ligand presentation. Polysulfation is used to mimic the heparan sulfate-containing surface of cells and to compete with this natural binding site of viruses. Here, orthopoxvirus strains are used as model viruses as they use heparan sulfate for cell entry. Scanning force microscopy (SFM) showed that the dPGs were successfully grafted to the TRGO sheets, resulting in abundant presence of dPG on the sheets. The newly designed graphene derivatives demonstrate excellent binding as well as efficient inhibition of orthopoxvirus infection.