Dresden 2017 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 111: Nanostructures at Surfaces: Molecular Systems III
O 111.10: Vortrag
Freitag, 24. März 2017, 12:45–13:00, REC/PHY C213
Thermal Fluctuations of Structured Adhered Bio-Membranes Probed with Nanometer/Microsecond Resolution — •Cornelia Monzel1,2,3, Daniel Schmidt4,5, Ana-Suncana Smith4, Udo Seifert5, Kheya Sengupta2, and Rudolf Merkel1 — 1Forschungszentrum Jülich, Germany — 2Centre Interdisciplinaire de Nanoscience de Marseille, France — 3present address: Institut Curie, France — 4Friedrich-Alexander Universität Erlangen-Nürnberg, Germany — 5Universität Stuttgart, Germany
Bio-membrane adhesion is essential for life and understanding its physical basis is key for further insight into many vital processes. Here, we explore bio-membrane adhesion to heterogeneously distributed linkers, as mimicked by micropatterns of adhesive and nonadhesive regions. As bio-membrane, we use closed lipid shells, so-called giant unilammellar vesicles (GUV) which bind to the patterned linkers, but, intriguingly, exhibit a fluctuating membrane within nonadhesive regions. The thermally excited fluctuations result in an entropic repulsion spatially separating the membrane from the substrate. Additional attractive and repulsive interactions near the substrate determine the final shape of the membrane, which we explore using different adhesion pattern and applying osmotic pressure changes. We further advance Reflection Interference Contrast Microscopy and develop Dynamic Optical Displacement Spectroscopy to monitor membrane undulations with up to 4nm and 10−6s resolution. From the analysis of this data and by expanding the theoretical framework a coherent view of this structured adhered membrane and its surface interaction is obtained.