Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 1: Protein Structure and Dynamics
BP 1.10: Vortrag
Montag, 12. März 2018, 12:15–12:30, H 1028
High spatial and temporal resolution study of biological processes in a live cell via interferometric scattering microscopy (iSCAT). — Richard Taylor1, Reza Gholami1, Verena Rauschenberger2, •Anna Kashkanova1, Alexandra Schambony2, and Vahid Sandoghdar1 — 1Max Planck Institute for the Science of Light, Erlangen, 91058, Germany — 2Developmental Biology Unit, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, 91058, Germany
Transmembrane proteins on a live cell exhibit a variety of dynamic behaviors, such as diffusion on the cell membrane, transport into the cell and trafficking along filaments. Those processes have been studied using standard technologies, however real-time visualization with nanoscopic resolution is a challenge, with fluorescent microscopy as the primary workhorse.
However, fluorescent microscopy has a critical limitation: fluorophores emit a limited number of photons in their lifetime, which limits spatial and temporal resolution, and the observation time. Interferometric scattering microscopy (iSCAT), uses gold nanoparticles in place of fluorophores. The scattered light is imaged interferometrically and the measurement can be performed indefinitely with spatial (temporal) resolution of several nanometers (microseconds).
We present an experiment in which the epidermal growth factor receptor (EGFR) protein in a live HeLa cell was labeled with a 50 nm gold nanoparticle and its life cycle observed. Our precise 3D information provides exciting new insights into the dynamics of the receptor.