Berlin 2018 – wissenschaftliches Programm
BP 10.23: Poster
Montag, 12. März 2018, 17:30–19:30, Poster C
Near Real Time Analysis of Stress Fiber Formation in Stem Cells — •Lara Hauke, Carina Wollnik, and Florian Rehfeldt — University of Göttingen, Third Institute of Physics - Biophysics
Human mesenchymal stem cells (hMSC) can be directed to differentiate into various lineages by different matrix elasticities. While changes in lineage specific protein expression occur over a period of days to weeks, significantly different structures of stress fibers are observable within the first 24 hours of plating  quantified by an order parameter S. With our massively parallel live-cell imaging set-up we record cells under physiological conditions (37 ∘C, 5 %CO2) over a period of 24-48 hours to obtain a statistically sufficiently large data set. We aim for a full representation of filament processes over time and space allowing for statistical analysis. This unbiased classification will be represented by persistence in space and time and potential cross-talk with other cytoskeletal components. For this we developed the *FilamentSensor* [2,3] a freely available tool for near real-time image analysis of stress fibers. We present experimental data where we can distinguish the development of hMSCs on 1 kPa, 10 kPa and 30 kPa elastic substrates with 99 % confidence and are working on single filament tracking and better analysis of orientation fields. References: A. Zemel, et al., Nat. Phys., 2010. www.filament-sensor.de B. Eltzner, et al., PLoS One, 2015.