Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 11: Bioimaging and Biopspectroscopy I
BP 11.11: Vortrag
Dienstag, 13. März 2018, 12:30–12:45, H 1028
Optical Mapping of Contracting Hearts — •Johannes Schröder-Schetelig1, Jan Christoph1,2, and Stefan Luther1,2 — 1Max Planck Institute for Dynamics and Self-Organization, Göttingen, Germany — 2German Center for Cardiovascular Research (DZHK e.V.), Göttingen, Germany
Optical mapping of isolated, intact hearts or myocardial cell cultures using fluorescent dyes has become a very well established tool in cardiac research. However, the method as such can be very sensitive to movement of the sample, resulting in severe motion artifacts in the recorded optical signals. In order to prevent this, in the past either the contraction had to be suppressed or more sophisticated strategies like ratiometric imaging had to be applied.
Here, we present a new method, which combines marker-free 2D video tracking techniques with panoramic optical mapping of freely beating and contracting Langendorff-perfused hearts using multiple calibrated cameras. We find that it is possible to accurately track and reconstruct the 3D deformation of the cardiac surface. The tracking is achieved without the need for additional landmarks or special patterned lighting schemes. By projecting the fluorescence videos directly onto the deforming mesh geometry, motion artifacts become significantly reduced. This opens up a new way, where the contraction of a heart is not considered a disturbing limitation any longer, but is now a property which can be measured and studied.