Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 11: Bioimaging and Biopspectroscopy I
BP 11.3: Vortrag
Dienstag, 13. März 2018, 10:15–10:30, H 1028
High resolution imaging of the drug delivery into stratum corneum of human skin probed with scanning near-field optical microscopy — •P. Patoka1, G. Ulrich1,2, K. Yamamoto1, A. Klossek1, F. Rancan3, A. Vogt3, U. Blume-Peytavi3, P. Schrade4, S. Bachmann4, G. Ulm2, B. Kästner2, and E. Rühl1 — 1Physical Chemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin — 2Physikalisch-Technische Bundesanstalt (PTB), Abbestr. 2-12, 12587 Berlin — 3Klinisches Forschungszentrum für Haut- und Haarforschung, Charité Universitätsmedizin, 10117 Berlin — 4Abteilung für Elektronenmikroskopie at CVK, 13353 Berlin
Understanding the mechanism of topical drug delivery into human skin requires the use of multiple techniques. Among those techniques label free methods are of special interest, avoiding drug-labels or skin-label interactions. Scanning near-field optical microscopy can be used to obtain detailed information on the correlation of the local drug distribution with highly resolved topographical information. Recent results from optical near-field microscopy imaging, investigating the penetration of the anti-inflammatory drug dexamethasone in human skin, are reported.
After resonant excitation of dexamethasone by a quantum cascade laser, operating in the mid-infrared regime, the penetration of dexamethasone in the stratum corneum is visualized. Imaging with high spatial resolution of <10 nm gives access to detailed information of the local drug distribution within the lipid matrix of the stratum corneum and its substructures. By using this technique also the presence of natural corticosteroids within the stratum corneum and ceramides is revealed. These measurements can be correlated with recent results obtained from X-ray microscopy and high resolution electron micrographs allowing us to reach an improved understanding of the drug penetration in human skin using label-free spectromicroscopy.