Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 16: Membranes and Vesicles I (joint session BP/CPP)

BP 16.12: Vortrag

Mittwoch, 14. März 2018, 12:45–13:00, H 1028

Formation and phase transitions of vapour deposited phospholipid bilayers on porous silicon substratesNicolas Moraga1, Marcelo Cisternas1, Diego Diaz1, Rodrigo Catalan1, Maria J. Retamal2, Tomas P. Corrales3, Mark Busch4, Patrick Huber4, Marco Soto-Arriaza2, and •Ulrich G. Volkmann11Institute of Physics and CIEN-UC, P. Univ. Catolica de Chile, Santiago, Chile — 2Faculty of Chemistry and CIEN-UC, P. Univ. Catolica de Chile, Santiago, Chile — 3Department of Physics, UTFSM, Valparaiso, Chile — 4TUHH, Hamburg, Germany

Study of phospholipid artificial membranes on solid substrates has become a relevant way to gain insight into the physical behaviour of cell membranes. In this work, porous silicon substrates (pSi) were made using a two-electrode cell to produce different pore diameters. Substrates were characterized with Field Emission Electron Microscopy. The phospholipid (DPPC) was deposited in high vacuum from the gas phase on the pSi. Film thickness was controlled using Very High Resolution Ellipsometry (VHRE). Samples were hydrated in air with ultrapure water to assemble the bilayer. Phase transitions were measured with VHRE and Stray Light Intensity during temperature cycles. AFM was used to study morphological changes of bilayers as a function of temperature. Our results open new ways to hydrate lipid bilayers using pSi with a specific pore diameter. Acknowledgements: Postdoctoral FONDECYT #3160803 (MJR), FONDECYT #1141105 (UGV) and #1171047 (MSA), FONDECYT INICIACION #11160664 (TPC), CONICYT Fellowships (RC, MC) and CONICYT-PIA ACT 1409.

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