Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 16: Membranes and Vesicles I (joint session BP/CPP)

BP 16.5: Vortrag

Mittwoch, 14. März 2018, 10:30–10:45, H 1028

Conditions of Spontaneous Translocation of Individual Nanotube Porin Through a Phospolipid BilayerYachong Guo1,2, Marco Werner2, Ralf Seemann3, Vladimir Baulin2, and •Jean-Baptiste Fleury31Nanjing University, Nanjing , China — 2Universitat Rovira i Virgili, Tarragona, Spain — 3Saarland Univsersity, Saarbruecken, Germany

Single ultra-short nanotubes can be inserted in cell membrane to be used as a membrane nanosensor or to form artificial ionic channels. Recent studies reported that ultra-short nanotubes can passively be inserted perpendicularly to the lipid bilayer core. After this insertion, it is commonly expected that these ultra-short nanotubes should stay trapped into the lipid bilayer core as its represents a potential well. In contrast to such expectations, we investigate the possible conditions that could lead a single nanotube to translocate spontaneously across a lipid bilayer. We demonstrate that membrane stretching and subnanometer nanotube, are essential to enable this type of translocation, while no translocations are occurring in lipid bilayers under low tension. The proof of this tension-dependent translocation event is obtained by observating directly a single nanotube quitting a highly stretched lipid bilayer. A quantitative analysis of the kinetic pathway associated to this translocation event is measured by using a specially designed microfluidic device combining optical fluorescence microscopy with simultaneous electrophysiological measurements.

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