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Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 17: Cell Mechanics I

BP 17.1: Vortrag

Mittwoch, 14. März 2018, 09:30–09:45, H 1058

Mechanical and Electrical Characterization of Cardiac and Skeletal Muscle Microtissues — •Delf Kah, Ingo Thievessen, Marina Spörrer, Wolfgang H. Goldmann, and Ben Fabry — Department of Physics, Biophysics Group, Friedrich-Alexander-University Erlangen-Nuremberg, D-91052, Erlangen, Germany

In-vitro engineered muscle tissue grafts are of growing interest for different applications including regenerative therapy, replacement of infarcted cardiac sites, or as a drug testing platform. Critical for the successful development of suitable models for engineered muscle grafts is the maturation into an in-vivo-like, highly aligned, and contractile tissue. To achieve this, we developed a stretchable and electrically paceable system consisting of an array of 4x2x2 mm microwells with two elastic pillars that serve as force sensors. Tissues can be grown from several cell sources including neonatal cardiomyocytes form rats, mice, as well as C2C12 skeletal muscle cells. Mechanical stretching with a linear stepper motor, electrical pacing with carbon electrodes, and microscopic imaging of the tissue is synchronized by a microcontroller, allowing us to study isotonic, isometric, or eccentric contractions for various pacing protocols. Cardiac tissues show remarkably uniform contraction induced by electrical pacing, which allows for imaging with a time resolution of up to 1000 Hz through heterodyning. Accordingly, contractile performance can be evaluated with high temporal precision.

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