Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 2: Biomaterials and Biopolymers (joint session BP/CPP)
BP 2.8: Vortrag
Montag, 12. März 2018, 11:45–12:00, H 1058
Small-angle X-ray scattering on gold nanoparticle-decorated DNA-origami nanostructures — •Kilian Frank1,2, Caroline Hartl1, Amelie Heuer-Jungemann1, Tim Liedl1, and Bert Nickel1 — 1Faculty of Physics and Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, Geschwister-Scholl-Platz 1, 80539 München, Germany — 2present address: Georg-August-Universität, Institute for X-ray Physics, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany
The DNA origami technique is a robust method for positioning guest molecules at the nanoscale, allowing for 3D crystalline assembly from monomeric building blocks. We report on synchrotron small-angle X-ray scattering (SAXS) experiments on DNA origami with guest gold nanoparticles. Geometric models were applied to investigate the particle placement and the lattice parameters of crystalline superstructures. In collaboration with Heinz Amenitsch (TU Graz) the model-free pair distance distribution function (PDDF) from the scattering data was analyzed. The PDDF reveals interparticle distances with nanometer resolution and is thus a valuable tool in the study of DNA-templated particle assemblies. The structure of a DNA-based lattice was confirmed to be rhombohedral with a spacing of 65 nm (T. Zhang, C. Hartl, S. Fischer, K. Frank, P. Nickels, A. Heuer-Jungemann, B. Nickel and T. Liedl. arXiv: 1706.06965). In situ SAXS confirmed previously found melting temperatures of the structures. These results help to optimize future designs of monomeric building blocks regarding lattice type and size.