Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 28: Single Molecule Biophysics
BP 28.9: Vortrag
Donnerstag, 15. März 2018, 12:00–12:15, H 1058
Detection of nanoscale biological samples using Nanocapillaries — •Tobias Jäckering1, Marco Radukic2, Dario Anselmetti1, and Martina Viefhues1 — 1Experimental Biophysics, Physics Faculty, Bielefeld University, Bielefeld, Germany — 2Biotechnological Faculty, Bielefeld University, Bielefeld, Germany
Filamented borosilicate glass capillaries can be pulled down to 40 nm diameter nanopipettes with the ability of easy filling the nanopipette via capillary forces. These nanopipettes were used for the detection and analysis of nanometer sized biological analytes by monitoring the electric (ionic) current during translocation through the nanopipette. We applied either electrophoretic or hydrodynamic driving forces to investigate different types of analytes like DNA and adeno-associated viruses (AAV).
To differentiate the detected signal from the background it is essential to adjust the nanopore-size of the nanopipette to the respective analytes size. Additionally the respective buffer has a significant effect on the measurement. We will demonstrate that these nanoscopic coulter-counter experiments allow to distinguish various analytes like DNA and AAV.