Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 6: Cytoskeletal Filaments I
BP 6.9: Vortrag
Montag, 12. März 2018, 17:15–17:30, H 1058
Investigations on the cell morphology of oral mucosa cancer and non-cancer cells — •Nina Bartels1, Maja Strugacevac1, Constanze Wiek2, Julia Kristin2, Marcel Glaas2, Jörg Schipper2, and Mathias Getzlaff1 — 1Heinrich-Heine-Universität Düsseldorf, Institute of Applied Physics, Universitätsstr. 1, 40225 Düsseldorf, Germany — 2Düsseldorf University Hospital, Department of Othorinolaringology, Moorenstrasse 5, 40225 Düsseldorf, Germany
In order to develop new cell-selective treatment strategies for head and neck squamous cell carcinoma, our group is investigating the differences of the cell morphology and physical properties of different oral cancer cells and oral keratinocytes. The cell lines originate from different locations of the oral mucosa and are investigated using a fluorescence microscope.
To obtain more information about cell morphology, the cells were stained using CellMask Green (cell membrane) and Hoechst (cell nuclei) staining kits. By the confocal laser-scanning microscope three-dimensional images of the cells were made to compare the different cell lines in size, volume and shape.
A staining kit for active mitochondria (MitoTracker) enables us to compare the aerobic metabolism of tumor and non-cancer cells to verify the Warburg hypothesis. Additionally, actin filaments and microtubules were stained to observe differences in the cytoskeleton which is specific for cell elasticity. This contribution will show and discuss our latest results.