Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 9: Postersession I

BP 9.24: Poster

Montag, 12. März 2018, 17:30–19:30, Poster A

Exposure of mesenchymal stromal cells to graphene quantum dots — •Sibel Tezkan1, Stefan Fasbender1, Tim Ebbecke2, Katharina Raba2, Johannes Fischer2, and Thomas Heinzel11Experimental Condensed Matter Physics, Heinrich Heine University, Düsseldorf — 2Transplantation Diagnostics and Cell Therapeutics, University Hospital Düsseldorf

Fluorescent graphene quantum dots (GQDs) are prepared by the slightly modified method of Qu et al. [1] via thermal decomposition of citric acid and diethylenetriamine with subsequent dialysis to obtain a pure GQD solution. The obtained aqueous solution is analyzed with fluorescence spectroscopy. Mesenchymal stromal cells are exposed to GQDs and a high uptake is determined using flow cytometry. The number of incorporated GQDs is estimated by comparing the fluorescence of cells with GQDs and without GQDs. In addition, the uptake of GQDs into exosomes is investigated.

[1] Qu et al., Light: Science & Applications, 2015, 4, e364

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DPG-Physik > DPG-Verhandlungen > 2018 > Berlin