Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 9: Postersession I
BP 9.4: Poster
Montag, 12. März 2018, 17:30–19:30, Poster A
Analysis of dynamics of membrane-protein microdomains in bacteria — Daniella Lucena1,3, •Marco Mauri1,2, Felix Schmidt1,4, Bruno Eckhart1,4, and Peter L. Graumann1,3 — 1SYNMIKRO, Marburg — 2INRIA Grenoble, France — 3Department of Chemistry, Philipps University, Marburg — 4Department of Physics, Philipps University, Marburg
Cell membrane has a remarkably intricate temporal and spatial organization that is central for the maintenance of fundamental processes in bacteria. While early models often envisioned proteins freely and homogeneously diffusing on the membrane, there is nowadays growing evidence in support of lipid microdomains. To date there has been no broad study to evaluate how protein size, number of transmembrane domains, and temperature affect the diffusion of membrane proteins. In this work, we have undertaken a systematic study of the effects of these factors on membrane protein diffusion and investigate the dynamics of membrane organization in live B. subtilis cells by mean of single-molecule tracking, physical modelling and computer aided visualization methods. We found that diffusion coefficients do not correlate with protein molecular weight, but decrease with increased transmembrane radius. Moreover, diffusion coefficients are anomalous and are better described by discriminating diffusion rates into two protein populations. Also, we observed that temperature can influence the spatiotemporal organization of membrane proteins and significantly impact their dynamics. We think that data analysis methods here introduced can be valuable for membrane protein studies in any bacteria.